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. 2016 Apr 26;10:30. doi: 10.3389/fncir.2016.00030

Figure 8.

Figure 8

The larval vENT contains glutamatergic and calretinin-positive populations of cells. (A,A″) Confocal images of Tg(lhx5:GFP)b1205/Tg(slc17a6b:DsRed)nns9Tg 4 dpf double transgenic larvae labeled with anti-GFP and anti-DsRed antibodies (green and magenta respectively). GFP expression shows the previously identified vENT (orange box in A, enlarged in A′,A″). Enlarged views of single confocal sections (A′,A″) show co-expression of GFP and DsRed in a subpopulation of cells (arrowheads) of the GFP+ vENT (dotted line in A′ marks). (B–C′) Tg(lhx5:GFP)b1205 4dpf larvae labeled with anti-GFP (green) and anti-calretinin (magenta). (B,B′) lateral view shows a subset of GFP+ vENT neurons (bound by orange box) express calretinin. (C,C′) 3D transverse sections of same larvae (B) cropped to area delineated by red box in (B). Calretinin+ and GFP+ neurons within the vENT, which surrounds the lateral forebrain bundle (lfb; asterisk). Scale bars: (A,B) 100 μm; (A′,C) 50 μm.