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L3-3B inhibition of intracellular RANKL signaling. BMM cells were serum-starved for 5 h and stimulated for 10 min with (A) RANKL (1 μg/ml) or (B) M-CSF (50 ng/ml). L3-3B (1 μg/ml) was pre-incubated with RANKL or M-CSF for 30 min prior to stimulation. Whole-cell lysates were harvested and immunoblotted with indicated antibodies.
