Skip to main content
. 2016 Feb 26;39(4):322–329. doi: 10.14348/molcells.2016.2292

Fig. 5.

Fig. 5.

CaV2.2 currents were suppressed by depletion of PI(4,5)P2. TsA201 cells were co-transfected with CaV2.2 channels, Lyn11-FRB (plasma membrane anchoring protein), and one of the following four constructs: PJ-Dead, PJ-Sac, INPP5E, or PJ. Rapamycin was applied for 60 s. (A) Time courses of CaV2.2 currents in cells expressing PJ-Dead, PJ-Sac, INPP5E, or PJ. (B) Summary graph of % inhibition by rapamycin addition in CaV2.2 currents (n = 6 for PJ-Dead; n = 9 for PJ-Sac; n = 5 for INPP5E; and n = 11 for PJ). (C) Summary graph of the time constant for rapamycin-induced inhibition in CaV2.2 currents (n = 9 for PJ-Sac; n = 5 for INPP5E; and n = 11 for PJ). Data are mean ± SEM. * P < 0.05, ** P < 0.01, and *** P < 0.001, with one-way ANOVA followed by Bonferroni post-hoc test.