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Inhibition of normal head and axis formation by depletion of endogenous xCyp26c. Morpholino activity was confirmed by Western blot. (B) Embryos injected with 40 ng of MO-xCyp26c did not have head structures and had short axis formation. (C) xCyp26c was injected with DNBR and animal caps were cultured to stage 24. Expression patterns of Otx2 and HoxB9 were analyzed by RT-PCR.
