. 2016 Apr 26;6:24966. doi: 10.1038/srep24966

Table 1. Primers for the PCR-screen of nitrifying functional genes and 16S rRNA sequences and their amplifying results.

Primer	Sequence (5′-3′)	Target gene	Ta (°C)	Approach	No. of DNA clones	No. of transcript clones	Reference
340F	CCCTAYGGGGYGCASCAG	Archaea 16S rRNA	57	PCR	69	65	9
1000R	GGCCATGCACYWCYTCTC	Archaea 16S rRNA	57	PCR	69	65	9
356F	ACWCCTACGGGWGGCWGC	Bacteria 16S rRNA	60	PCR	204	187	15
1064R	AYCTCACGRCACGAGCTGAC	Bacteria 16S rRNA	60	PCR	204	187	15
amo111F	TTYTAYACHGAYTGGGCHTGGACATC	Archaea amoA	55	PCR	73	68	8
amo643R	TCCCACTTWGACCARGCGGCCATCCA	Archaea amoA	55	PCR	73	68	8
archamoAqF	CCRGTCTGGTTRCCDTCAGG	Archaea amoA	57	qPCR	–	–	This study
archamoAqR	CTTGAAYGCVGTYTCAAGCG	Archaea amoA	57	qPCR	–	–	This study
amoA1F	GGGGTTTCTACTGGTGGT	Bacteria amoA	50	PCR	N.A.	N.A.	12
amoA2R	CCCCTCKGSAAAGCCTTCTTC	Bacteria amoA	50	PCR	N.A.	N.A.	12
amoA3F	GGTGAGTGGGYTAACMG	Bacteria amoA-amoB	45	PCR	N.A.	N.A.	11
amoB4R	GCTAGCCACTTTCTGG	Bacteria amoA-amoB	45	PCR	N.A.	N.A.	11
nxrA1F	CAGACCGACGTGTGCGAAAG	Nitrobacter/Nitrococcus nxrA	55	PCR	N.A.	N.A.	14
nxrA2R	TCYACAAGGAACGGAAGGTC	Nitrobacter/Nitrococcus nxrA	55	PCR	N.A.	N.A.	14
nxrA0038F	GCCAGTGGGAAGAGTTCTATA	Nitrolancea nxrA	68	PCR	N.A.	N.A.	13
nxrA3595R	GCCACGTGCGTGTCCCGSGT	Nitrolancea nxrA	68	PCR	N.A.	N.A.	13
NpnnxrBF	TAYATGTGGTGGAACAAYGTRG	Nitrospina nxrB	54	PCR	N.A.	N.A.	This study
NpnnxrBR	GCCTTKCGMGGACAMGCCGC	Nitrospina nxrB	54	PCR	N.A.	N.A.	This study
nxrB169F	TACATGTGGTGGAACA	Nitrospira nxrB	55	PCR	16	27	10
nxrB638R	CGGTTCTGGTCRATCA	Nitrospira nxrB	55	PCR	16	27	10
nxrBqF	TGTGGTGGAACAACGTGGAA	Nitrospira nxrB	56	qPCR	–	–	This study
nxrBqR	CCCGGCATCGAAAATGGTCA	Nitrospira nxrB	56	qPCR	–	–	This study

N.A., PCR detection was not available; “–”, clone sequencing was not performed.