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. 2016 Apr 26;6:25133. doi: 10.1038/srep25133

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(a) Western blot of Y2H gold whole cell extract expressing the indicated plasmids using anti-myc antibody to detect various viral proteins (as GAL4 binding domain fusion). BD: GAL4 binding domain expression plasmid. (b) Western blot of Y2H gold whole cell extract expressing the indicated plasmids using anti-HA antibody to detect the expression of GAL4 activation domain fused X-domain. AD: GAL4 activation domain expression plasmid. (c) Quantitative α galactosidase assay of Y2H gold cotransformants represented in Table 1. Normalized α galactosidase units are plotted as ± SEM of triplicate samples. (d) CoIP of ORF3 (ORF3-myc) and X-domain (X-HA) expressing Huh7 cell extract, immunoprecipitated using indicated antibodies and immunoblotted using anti-myc (upper panel) and anti-HA (lower panel) antibodies. RS: Rabbit pre immune serum. (e) CoIP of methyltransferase (Met-Flag) and X-domain (X-HA) expressing Huh7 cell extract, immunoprecipitated using indicated antibodies and immunoblotted using anti-Flag (upper panel) and anti-HA (lower panel) antibodies. RS: Rabbit pre immune serum. (f) CoIP of helicase (Hel-myc) and X-domain (X-HA) expressing Huh7 cell extract, immunoprecipitated using indicated antibodies and immunoblotted using anti-myc (upper panel) and anti-HA (lower panel) antibodies. RS: Rabbit pre immune serum.