| Oligonucleotides for isogenic deletion mutants |
| essB-att1 |
GGGGACAAGTTTGTACAAAAAAGCAGGCTGATGGCGACAGACAAAACCAAGC |
| essB-rev1 |
CTATTTTTCCTCCTATAGTAACTTC |
| essB-rev2 |
GTTACTATAGGAGGAAAAATAGGTATAGGACTGAGGCAAAGACAATGC |
| essB-att2 |
GGGGACCACTTTGTACAAGAAAGCTGGGTGTGCCCATTTCAATGTTTCAAC |
| agr-att1 |
GGGGACAAGTTTGTACAAAAAAGCAGGCTACCCTTTCAATTGTCTGACG |
| agr -rev1 |
GATGAATAATTAATTACTTTCATTGTAAA |
| agr-rev2 |
AGTAATTAATTATTCATCACTTACCTATTTAACGTTTGTCTACA |
| agr-att2 |
GGGGACCACTTTGTACAAGAAAGCTGGGTGGGATGCCTTTATTGGTG |
| Oligonucleotides for genetic complementation |
| EssB-Sma1-F |
GAGCCCGGGAGATGGTTAAAAATCATAACCCTAAAAATG |
| EssB-BamH1-R |
GAGGGATCCCTATTTTTTTCTTTCAGCTTCTT |
| Oligonucleotides for qRT- PCR |
| gyrB-F |
CAAATGATCACAGCATTTGGTACAG |
| gyrB-R |
CGGCATCAGTCATAATGACGAT |
| essB-F |
TTTTCACAAGAGATGGATTACCAATT |
| essB-R |
TTCTGACACCGGTAATGGATCA |
| esxA-F |
CGTGCACAAGGTGAAATTG |
| esxA-R |
CGAAACGGCTGAAAGCTT |
| essA-F |
AAAAGCCCCTATATTCAAAACAA |
| essA-R |
GCCCCTACAGACATCAAAATG |
| essC-F |
TGCGTGCCTTAACATCAATC |
| essC-R |
TAACGTCATGCTCTCCGAATAA |
| esaA-F |
GTCAAGATGAACCAAATGATAAGAA |
| esaA-R |
CCATCAAGGTTATGATTCAATGTT |
| esaB-F |
CAAATTAAAGTGATGACGAAAGGT |
| esaB-R |
CTCCATCAGCGATTTGATAATC |
| Oligonucleotides for construction and purification of GST-tagged fusion protein |
| ESSB-BamH1-F |
GAGGGATCCATGGTTAAAAATCATAACCCTAAAAATG |
| ESSB-EcoR1-R |
GAGGAATTCCTATTTTTTTCTTTCAGCTTCTT |
