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. 2016 Apr 26;6:25096. doi: 10.1038/srep25096

Figure 3. Southern blot showed extra-chromosomally open circular and supercoiled topoforms of pGL3-35S in different tissues on day 7 days in the muscle of Atlantic salmon.

Figure 3

M: DIG labelled DNA Molecular Weight Marker II (Roche), OC: open relaxed topoform, SC: supercoiled topoform, 1: kidney control DNA, 2: liver, 3: kidney, 4: spleen, 5: heart, 6: gill, 7: muscle, 8: anterior intestine, 9: posterior intestine, 10: injection site, 11: 100 pg of pGL3-35S. DNA from samples of five fish from each treatment groups were mixed to yield samples for the Southern blot experiment.