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. 2016 Apr 26;6:25096. doi: 10.1038/srep25096

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Samples shown were obtained 150 and 536 days after injection. M: DIG-labelled DNA Molecular Weight Marker II (Roche), OC: open circular topoform, L: linear topoform, SC: supercoiled topoform, 1: day 150, 2: day 536, 3: 100 pg of pGL3-35S. DNA from samples of five fish from each treatment groups were mixed to yield samples for the Southern blot experiment.