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. 2016 Apr 26;6:24610. doi: 10.1038/srep24610

Figure 5. TcMVK induces phosphorylation of HeLa cell cytoskeletal modulators.

Figure 5

(A) rTcMVK triggered time-dependent phosphorylation of Src (arrow), reaching a maximum phosphorylation at 90 min (asterisks). FAK displays increased TcMVK-dependent phosphorylation, noticeable at 5, 30 and 120 min (asterisks). PAK activation time-dependent increases up to 120 minutes. (B) rTcMVK induced the start of phosphorylation of MAPK components, ERK and p38, at 1 and 5 min, respectively. ERK displayed maximum activation at 30 min (asterisk), decreasing at 60 min and almost disappearing after 120 min. In contrast, p38 activation showed a time-dependent increase up to 120 min. Numbers under actin bands refer to the densitometric quantitation: protein/actin. Nt: negative control of cells without rTcMVK incubation. Arrows: time dependent increase; asterisks: phosphorylation peaks. Anti-actin was used as loading control. Band densitometry measurements were performed using UVIBAND®, UVITEC. Blots are representative of five independent experiments.