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. 2016 Apr 26;6:24993. doi: 10.1038/srep24993

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(a) Limiting dilution of sCJD-BH was used for RT-QuIC reaction. Fibril formations of recombinant PrP were visualised by measurement of ThT fluorescence. (b) Curve of seeding dose activities. Seeding dose 50% (SD₅₀) per gram was 10^10.5. (c) Limiting dilution of sCJD-BH was used for wire-QuIC reaction.