Table 5.

Application of 70 compound method with a protein precipitation step for fly bodies and heads. The average of 3 repeated injections with standard deviation is reported below. Values for analytes were reported only if they were above the LoD.

Analyte	Amount (pmol/fly)		Analyte	Amount (pmol/fly)
	Fly Bodies	Fly Heads		Fly Bodies	Fly Heads
ACh	2.64 ± 0.08	0.212 ± 0.011	Thr	18.4 ± 1.8	0.98 ± 0.04
Ch	312 ± 8.8	66.36 ± 1.14	VMA
CA			Trp	17.1 ± 0.9	0.44 ± 0.02
His	2.7 ± 0.1	0.27 ± 0.04	MOPEG
Ans			Kyo
Carn			Cys
HTau	21 ± 6		KA	1.9 ± 0.1	0.4 ± 0.04
Tau	278.5 ± 4.1	88.81 ± 3.19	Spd	0.03 ± 0.002	0.01 ± 0.0003
Arg	0.45 ± 0.01	0.06 ± 0.004	PhEt	0.336 ± 0.015	0.005 ± 0.0004
Hist	1.92 ± 0.13	0.772 ± 0.011	TrpA	1.85 ± 0.09	0.024 ± 0.002
Asn	6.5 ± 0.7	0.74 ± 0.04	NAS	29.61 ± 0.7	4.24 ± 0.242
Ser		0.8 ± 0.4	5HIAA	0.47 ± 0.07	0.025 ± 0.001
Gln	54.99 ± 0.59	5.61 ± 0.14	5HTOL
HSer	4.5 ± 1.1	0.4 ± 0.01	HCY
Cit			3HAA
ETA	44.03 ± 0.92	5.56 ± 0.27	HCA	0.07 ± 0.01
Asp			HVA
Agm			DOMA
Glc	3170 ± 295	1111.5 ± 0.2	Kyn	8 ± 0.7
Gly	8 ± 1	0.8 ± 0.004	Spm
Glu	1.1 ± 0.2	0.21 ± 0.03	DOPEG
BAla	83.5 ± 0.7	3.04 ± 0.13	5HTP	184.1 ± 3.6	1.83 ± 0.04
Ala	90.4 ± 5.4	22.84 ± 2.69	OA		0.003 ± 0.0002
NAP	0.16 ± 0.01	0.009 ± 0.0001	NM
GABA	6.25 ± 0.18	1.661 ± 0.055	Tyr	8.4 ± 0.2	0.35 ± 0.007
Pro	614.3 ± 2.8	100.46 ± 2.2	3HK	36 ± 3	1.7 ± 0.1
Ado	31.1 ± 0.12	2.599 ± 0.038	Syn
Val	22.3 ± 0.3	2.17 ± 0.1	5HT	81.66 ± 10.34	5.98 ± 0.666
Met	16.17 ± 0.58	1.186 ± 0.025	DOPAC	0.09 ± 0.003	0.013 ± 0.001
Orn			3MT
GSH			LDOPA	1.5 ± 0.13	0.541 ± 0.026
Lys	0.2 ± 0.02		TyrA	11.89 ± 0.64	0.062 ± 0.001
Put	0.08 ± 0.001	0.0074 ± 0.0003	NE
Leu	53.6 ± 1.9	3.84 ± 0.04	E
Phe	18.4 ± 0.3	1.02 ± 0.03	DA	0.22 ± 0.01	0.005 ± 0.0001