Fig. 1.

The effect of pectinase-treated Panax ginseng (GINST) on the viability of GC-2 sperm cells. The protocol described in the Materials and methods section 2.2 was followed. Cell viability was evaluated using the MTT assay. (A) The effect of GINST (50 μg/mL, 100 μg/mL, and 200 μg/mL) on the viability of GC-2 cells is shown. (B) The effect of GINST (50 μg/mL, 100 μg/mL, and 200 μg/mL) in GC-2 cells exposed to 200 μM hydrogen peroxide. The results are shown as percentage of the control samples. The data are expressed as the mean ± standard deviation (n = 6). * p < 0.01 compared with the control cells. ** p < 0.05 compared with the hydrogen peroxide-exposed cells as determined with Student t-test and one way analysis of variance using GraphPad Prism version 4.0 for Windows. GINST, pectinase-treated Panax ginseng extract; NS, not significant.