Fig. 3.

The effect of pectinase-treated Panax ginseng extract on the inhibin-α mRNA expression level in hydrogen peroxide-exposed GC-2 cells. The protocol described in the Materials and methods section 2.6 was followed. (A) The mRNA expression level of inhibin-α is shown. glyceraldehyde 3-phosphate dehydrogenase was used as an internal control. (B) The polymerase chain reaction band intensity of inhibin-α was analyzed using the ImageJ 1.41o software package and was normalized to that of glyceraldehyde 3-phosphate dehydrogenase. The data are expressed as the mean ± standard deviation (n = 6). * p < 0.01 compared with the control cells. ** p < 0.05 compared with the hydrogen peroxide-exposed cells. *** p < 0.01 compared with the hydrogen peroxide-exposed cells as determined with Student t-test and one way analysis of variance using GraphPad Prism version 4.0 for Windows. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GINST, pectinase-treated Panax ginseng extract.