Figure 4.

HTS results from Life Chemicals antimicrobial/antiviral focused library and hit validation. (A) Schematic of HTS with 25 000 Life Chemicals compounds and hit validation process. (B) Bar graphs of IC₅₀ values and the dissociation equilibrium constants (K_D) of six hit compounds determined by fluorescence-based enzymatic assay and Surface Plasmon Resonance (SPR), respectively. All data were normalized for immobilization levels of target proteins and reference. Bars that reach the top of the graph represent either IC₅₀ or K_D values of over 200 μM (no inhibition or no binding).