Extended Data Figure 8. Senescent cell removal does not impact somatotrophic axis signaling in vivo.
a, Glucose levels following intraperitoneal glucose administration following an overnight fast in 18-month-old vehicle- and AP-treated ATTAC C57BL/6 females. b, Normalized glucose levels following intraperitoneal insulin administration following a 4 hour fast in 18-month-old vehicle- and AP-treated ATTAC C57BL/6 females. c, Serum Igf1 levels in ATTAC C57BL/6 mice (n = 4 mice of each group). d, Representative western blots for phospho-S6K, total S6K, phospho-AKTS473 and total AKT in iWAT, kidney and skeletal muscle tissue lysates from 18-month-old vehicle- and AP-treated ATTAC C57BL/6 females. Serum Igf1 levels in ATTAC C57BL/6 mice (n = 4 mice of each group). e, Quantification of phospho-S6K to total S6K ratio in blots from (d), n = 4 mice of each group. f, Quantification of phospho-AKTS473 to total AKT ratio in blots from (d), n = 4 mice of each group. Error bars indicate s.e.m. No statistically significant differences were observed in a–c and e–f using unpaired two-tailed t tests. For gel source data, see Supplementary Fig. 1.