Extended Data Figure 10. Impact of senescent cell clearance on wound healing and tissue fibrosis.

a, Closure of 3-mm punch biopsy wounds in 18-month-old ATTAC females after treatment with vehicle or AP for 6 months and if drug treatment was stopped 2 days prior to skin puncture or continued during wound closure (n = 6 wounds for –AP;–AP and +AP;–AP and n = 10 wounds for –AP;+AP and +AP;+AP). AP administration during the wound healing process significantly attenuates the rate of wound closure independently of whether senescent cell removal had occurred prior to wounding. b, Closure of 3-mm punch biopsy wounds in 4-month-old ATTAC females after treatment with vehicle or AP following wounding (n = 10 wounds per group). Similar to 18-month-old mice, AP administration during the wound healing process dramatically attenuated the rate of wound closure. c, Quantification of total GFP⁺ cells isolated from 3-mm punch biopsy wounds of 4-month-old mice two days into the wound healing process treated with vehicle (black) or AP (red, n = 3 mice per group). d, PTAH-stained tissues sections from 18-month-old ATTAC mice for detection of fibrosis. Scale bars, 100 μm. Error bars indicate s.e.m. Unpaired two-tailed t tests were used to determine statistical significance for a–c. Mice receiving AP during the healing process in a and b are significantly different from those treated with vehicle from day 1.5 through day 9.5. *, P<0.05.