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. Author manuscript; available in PMC: 2016 Aug 3.
Published in final edited form as: Nature. 2016 Feb 3;530(7589):184–189. doi: 10.1038/nature16932

Extended Data Figure 3. ATTAC-mediated clearance of senescent cells is partial and tissue selective and attenuates expression of inflammation markers.

Extended Data Figure 3

a, Expression of the ATTAC transgene and a senescence marker panel, as determined by RT-PCR, in gastrocnemius, eye, kidney, heart (atria), spleen, lung, liver, and colon (n = 4 females per group). b, Expression of Il6, Il1α and Tnfα as determined by qRT-PCR in mouse iWAT, kidney and skeletal muscle at different ages (n = 4 females per group). Il6 values are as indicated in Extended Data Fig. 1d (iWAT) and 3a (kidney and gastrocnemius). Expression levels of inflammation markers in unmanipilated 18-month-old C57BL/6 females suggests that repeated vehicle injections were not a source of tissue inflammation. Error bars indicate s.e.m. Statistical significance was determined by unpaired two-tailed t tests. *, P<0.05; **, P<0.01; ***, P<0.001. Asterisks above individual bars in (a) denote significant changes to 2-month-old mice; asterisks above brackets denote significant differences between 18-month-old vehicle and AP treated mice.