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. 2015 Nov 13;4(11):e002553. doi: 10.1161/JAHA.115.002553

Figure 2.

Figure 2

Matrix metalloproteinase 2 deficiency is associated with abnormalities in lipid storage and transport. A, Oil Red O stain of neutral lipid species from frozen liver sections of fasted mice. Scale bar=30 μm. B, Fasting levels of hepatic lipids in Mmp2 −/− vs WT mice as assessed by high‐performance liquid chromatography with in‐line evaporative light‐scattering detection. n=3 per genotype.*P<0.05 vs WT. C, Fasted TG and cholesterol levels in lipoparticle fractions of plasma separated by fast‐performance liquid chromatography (left). Traces correspond to pools of plasma from Mmp2 −/− and WT. D, Hepatic TGs were elevated in nonfasting mice. Gavage treatment with vehicle or pan–secreted phospholipase A2 inhibitor varespladib; normalized hepatic TG levels in nonfasting Mmp2 −/− mice. n=4 individual mice per genotype per group (or treatment). *P≤0.05 vs WT. † P≤0.05 vs Mmp2 −/− administered vehicle. CE indicates cholesteryl esters; FA, free fatty acids; FC, free cholesterol; HDL, high‐density lipoprotein; mAU, milliabsorbance units; LDL, low‐density lipoprotein; PC, phosphatidyl choline; PE, phosphatidyl ethanolamine; PI, phosphatidyl inositol; PS, phosphatidyl serine; SM, sphingomyelin; TG, triglyceride; VLDL, very low‐density lipoprotein; WT, wild type.