Figure 9.

TRAIL and FGF‐2‐inducible proliferation, migration, and tubule formation involves H₂O₂ and NO. A, TRAIL‐inducible proliferation and (B) migration involves H₂O₂ and NO. Growth quiescent HMEC‐1 cells were treated with PEG‐Catalase (PEG‐Cat; 200 U/mL) and l‐NAME (1 mmol/L) for 1 hour prior to 10 ng/mL TRAIL treatment. Cell counts were assessed 72 hours later and migration assessed 24 hours later. C, TRAIL‐inducible tubule formation involves H₂O₂ and NO. Serum‐starved HMEC‐1 cells were seeded in growth factor reduced Matrigel. Cells were treated with TRAIL (10 ng/mL) and PEG‐Cat (200 U/mL) or l‐NAME (1 mmol/L). Tubule formation was photographed and quantified 3 hours later. FGF‐2‐inducible (D) proliferation, (E) migration, and (F) tubule formation involves H₂O₂ and NO. Treatment with FGF‐2 was identical to above except that 50 ng/mL FGF‐2 was used. Data represent the combined results of at least 3 independent experiments. Results are expressed as mean±SEM; 1‐way ANOVA; *P<0.05, **P<0.01 and ***P<0.001. FGF‐2 indicates fibroblast growth factor‐2; HMEC‐1, human microvascular endothelial cell‐1; l‐NAME, l‐NG‐nitroarginine methyl ester; NO, nitric oxide; NT, no treatment; TRAIL, tumor necrosis factor–related apoptosis‐inducing ligand.