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. 2016 Apr 26;4:42. doi: 10.1186/s40478-016-0309-4

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© Yao et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Evaluation of potential remyelinating drugs in primary cultures of oligodendrocyte precursor cells (OPCs). a. Representative immunofluorescence (Olig2, red; MBP, green) of OPC cultures treated for 6 days with vehicle (DMSO), T3 (30 nM) as positive control, and indicated drugs (GC-1 30 nM; benztropine, clemastine and fasudil 1 μM; others 5 μM) (top). Percentage of MBP-positive cells, with cells graded for degree of maturation (mean ± S.E., n = 18 fields in three wells, ** P < 0.01) (bottom). b. Clobetasol concentration-dependence study for OPC cultures treated for 6 days (top). Percentage of MBP-positive cells (mean ± S.E., n = 6 fields in three wells, ** P < 0.01) (bottom)