Fig. 2.

Iressa and DAPT treatment inhibits the EGFR- and notch pathways abrogating downstream signaling and cell viability in vitro. a, b WB analysis of CPH36 and CPH047 cells treated with 5 µM iressa, 5 µM DAPT or a combination for 24 h. WB was investigated for expression of EGFR, EGFRvIII, Akt, p-Akt, Erk, p-Erk and Hes-1. GAPDH and tubulin serves as loading controls. c, d Cell viability of CPH036 or CPH047 cells following 7 days of treatment with iressa, DAPT or a combination in the indicated doses (mean ± SEM, n = 5). *p < 0.05, **p < 0.01, ***p < 0.001