Table 3.
Clinical sensitivity and specificity for methylation detection using overall survival as end point
| Sensitivity (%) | Specificity (%) | |
|---|---|---|
| Direct-MSP | 10.7 | 86.5 |
| Nested-MSP | 33.1 | 73.8 |
| Pyrosequencing 5 % threshold | 22.8 | 73.1 |
| Pyrosequencing 10 % threshold | 17.8 | 83.6 |
| Pyrosequencing 15 % threshold | 16.1 | 85.3 |
| Pyrosequencing 20 % threshold | 14.4 | 87.7 |
| Pyrosequencing 25 % threshold | 11.0 | 90.2 |
| MS-HRM | 15.4 | 87.7 |
Sensitivity was calculated as a/(a + b). Specificity was calculated as d/(c + d)
MSP methylation-specific PCR, MS-HRM methylation-sensitive high-resolution melting