Pattern count on raw data (A and B), after a rough preprocessing step (C, D and E) to discard empty patterns or patterns containing obviously more than 2 cells on the first frame of the sequence. Eventually, the number of pattern where a transition from two to three cell was detected automatically (F) or manually (G). While a lower number of event is selected automatically, more than 80 % of the events selected automatically were also part of the manual selection whatever the experimental condition