Table 2.

The sequence of the forward primer and reverse primer used for FQ-PCR assays

Name	Primer sense	Primer sequence (5′ → 3′)	Amplification product (bp)
GAPDH	Forward	5′-CCGAGGGCCCACTAAAGG-3′	116
	Reverse	5′-GCTGTTGAAGTCACAGGAGACAA-3′
Occludin	Forward	5′-GGGACAGAGCCTATGGAA-3′	191
	Reverse	5′-GGAAGCGATGAAGCAAAA-3′
Claudin-1	Forward	5′-CTGGCTTCGCTGGGATGGA -3′	100
	Reverse	5′-TGGCCTGAGCAGTCACGATGTT-3′
ZO-1	Forward	5′-GATGAGCGGGCTACCTTA-3′	126
	Reverse	5′-ATGGGAGCGAACTGAATG-3′