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. 2013 Feb;26(1):75–83. doi: 10.1089/vim.2012.0061

FIG. 2.

FIG. 2.

Immunization schema and post-vaccination antibody responses. (a) Vaccination and blood/tissue sampling time course. (b) Anti-CHIKV antibody measurement by ELISA. Sera were collected a week after the third immunization and the total IgG produced was measured in each group. Values represent the mean (±SD) of duplicate wells; p values (p<0.0085) indicate that the data are statistically significant between pEnv and pEnv+pnsP2 (i.e., the titer for pEnv + pnsP2 vaccinated animals is significantly higher. (c) IgG subtype ELISA analysis. The graph shows CHIKV-Env specific IgG1, IgG2a, IgG2b, and IgG3 antibody levels in mice that were vaccinated with pEnv or pEnv+pnsP2. Error bars represent standard deviation (±S.D) of duplicate wells. (d) IgG1 to IgG2a level binding ratios are shown for mice (n=5) immunized with pEnv or pEnv+pnsP2 intramuscularly, delivered through MID-EP. The values shown represent the mean (±S.D). The ratios were not significantly different.