Table 3.
Confirmation of Early Lyme Disease by Western Blotting
| Subject | Western Blot Positivitya (+/−) |
|||
|---|---|---|---|---|
| Pretreatment |
2 mo Posttreatment |
|||
| IgM (Bands) | IgG (Bands) | IgM (Bands) | IgG (Bands) | |
| 1 | – | – | – | − (41) |
| 2 | – | – | – | − (41) |
| 3 | − (41) | − (41) | − (41) | − (41) |
| 4 | – | – (39, 41, 45) | – | − (39, 41) |
| 5 | – | – | – | − (41) |
| 6 | – | – | – | – |
| 7 | − (23) | − (41) | − (23) | − (23, 39, 41, 45) |
| 8 | – | − (41) | – | − (41, 58) |
| 9 | + (23, 41) | − (23, 41, 45) | + (23, 41) | − (23, 41, 45) |
| 10 | – | – | − (23) | − (23, 39, 41) |
| 11 | − (23) | – | + (23, 41) | – |
| 12 | – | − (41) | – | − (41) |
| 13 | – | – | – | – |
| 14 | – | – | − (23) | − (23, 41) |
| 15 | − (23) | − (41, 45, 58) | − (23) | + (18, 41, 45, 58, 66) |
| 16 | − (23) | – | − (23) | – |
| 17 | – | − (41) | – | − (41) |
| 18 | – | − (41) | NT | NT |
| 19 | − (23) | − (41) | − (23) | − (41) |
| 20 | − (23) | − (23, 39, 93) | − (23) | − (23, 39, 41, 93) |
| 21 | + (23, 41) | − (31, 41, 45) | + (23, 41) | − (31, 41, 45) |
| 22 | – | − (18, 23, 39, 41) | − (23) | + (18, 23, 39, 41, 45) |
| 23 | – | – | – | − (41) |
| 24 | − (23) | − (23, 41) | + (23, 39, 41) | − (23, 39, 41) |
| 25 | − (23) | – | + (23, 41) | − (23, 41) |
| 26 | + (23, 39, 41) | − (23, 39, 41) | + (23, 39, 41) | − (23, 39, 41, 45) |
| 27 | – | – | – | – |
| 28 | + (23, 39, 41) | + (23, 39, 41, 45, 93) | NT | NT |
| 29 | + (23, 39, 41) | − (23, 39, 41, 45) | − (23) | − (39, 41) |
| Total, No. (%) | 5 (17) | 1 (3) | 6 (22) | 2 (7) |
Abbreviations: IgG, immunoglobulin G; IgM, immunoglobulin M; NT, not tested.
a Positive (+) result based on Centers for Disease Control and Prevention criteria for Western blot positivity: IgM at least 2 of the 23, 39, or 41 bands, and IgG at least 5 of 18, 23, 28, 30, 39, 41, 45, 58, 66, or 93 bands.