FIGURE 1.
Frequencies of Th9 cells and serum levels of IL-9 and IL-10 in different stages of HBV infection. Sequential gating strategy for Th9 identification (CD3+CD8–IL-9+IL-17–) using flow cytometry from freshly isolated PBMC (A). The percentage of Th9 cells and the serum level of IL-9 and IL-10 were analyzed in HC, CHB, HBV-LC, and ACLF groups (B), and in HBeAg-positive and HBeAg-negative groups (C) using the Mann–Whitney U test. ACLF = acute-on-chronic liver failure; CHB = chronic hepatitis B; HBV-LC = hepatitis B virus-related liver cirrhosis, HC = health controls, IL = interleukin; .