Annotated MS/MS spectrum for novel peptide with parent mass ~3666 Da, targeted for being specifically expressed in the lateral/dorsal region. The entire annotation explains 74.45% of the total intensity, including a very strong 7-residue tag, [1089.62]VTSDVLV[1863.22], with a complete doubly charged b ion series b10–b17 and a complete y ion series y17–y24. A BLAST search of the protein sequence did not get any good hits suggesting that this is a novel peptide. Another spectrum of the same peptide, unmodified and 2 amino acids longer, confirms the identification. The parent mass of this peptide, ~3841 Da, also shows dorsal localization (see Supplemental Figure 10, Supporting Information).