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. Author manuscript; available in PMC: 2016 Nov 23.
Published in final edited form as: Dev Cell. 2015 Nov 23;35(4):444–457. doi: 10.1016/j.devcel.2015.10.023

Figure 5. MPZL3 and FDXR Regulate and are Required for ROS Mediated Differentiation.

Figure 5

(A) Reactive oxygen species (ROS) levels in differentiating cultured keratinocytes as measured by DCF-DA fluorescence. Hydrogen peroxide (H202) was used as a measurement control. [*] indicates p <0.05. n = 5 biological replicates, mean +/− SEM is shown. (B) ROS levels with MPZL3 and/or FDXR KD as measured by DCF-DA fluorescence. [*] indicates p <0.05. n = 5 biological replicates, mean +/− SEM is shown. (C) ROS levels with forced MPZL3 and/or FDXR expression as measured by DCF-DA fluorescence. [*] indicates p <0.05. n = 5 biological replicates, mean +/− SEM is shown. (D) Quantitation of transcript levels of a panel of differentiation markers with KD of MPZL3 and FDXR by siRNA with either DMSO treatment or treatment with galactose oxidase (GAO). n = 3 biological replicates, mean +/− SD is shown. [*] indicates p <0.05, [**] p < 0.01, [***] p < 0.001. (E) Quantitation of transcript levels of a panel of differentiation markers with enforced expression of MPZL3 and FDXR with either DMSO treatment or treatment with EUK134. n = 3 biological replicates, mean +/− SD is shown. (H) Summary heatmap of the ROS rescue experiments. See also Supplemental Figure S5.