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. Author manuscript; available in PMC: 2016 Apr 26.
Published in final edited form as: Methods Mol Biol. 2015;1311:349–362. doi: 10.1007/978-1-4939-2687-9_23

Fig. 2.

Fig. 2

An example for designing an sgRNA that targets the mRFP gene. The PAM sequence (CCC) is underlined and bold. The base pairing sequence is in bold italics. The bottom DNA strand is the DNA transcription template strand