| Cell culture | 1 μL |
| Forward colony PCR primer, 10 μM | 0.5 μL |
| Reverse colony PCR primer, 10 μM | 0.5 μL |
| Taq Master Mix, 2× | 12.5 μL |
| ddH2O | 10.5 μL |
| Total | 25 μL |
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