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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Mol Microbiol. 2015 Dec 22;100(1):1–14. doi: 10.1111/mmi.13284

Figure 3. Suppression of ΔwecE shape defects.

Figure 3

(A) Shape. ΔwecE cells harboring plasmids carrying the indicated genes were grown to an OD600 = 0.5–0.6 at 37°C in LB containing 25 μM IPTG. Cells were fixed, then photographed by phase-contrast microscopy. The white bar represents 4 μm. (B) Flow cytometry. Histograms of the forward scatter area of live cells shown in panel A. The vertical dashed line represents the mean forward scatter area of the ΔwecE mutant containing a plasmid expressing wecE (blue graph). Mean of the forward scatter area is reported in arbitrary units (AU). Flow cytometry data is from 100,000 events (cells). Data is representative of two independent experiments. Strains: MAJ78 (vector), MAJ79 (wecE), MAJ92 (uppS), MAJ135 (bacA), MAJ181 (uppS), and MAJ238 (wbbL).