Figure 1.

Isoproterenol directs the differentiation of HAP cells into cardiac muscle cells. (A) Schematic diagram of inducing conditions for cardiac muscle cell differentiation from the upper part of vibrissa hair follicles. Hair follicles were cultured for 14 days in 3 conditions in DMEM medium with 10% fetal bovine serum (FBS): unsupplemented; supplemented with isoproterenol; or supplemented with the combination of isoproterenol+activin A+BMP4+bFGF; once every four days. (B) Immunostaining of cardiac muscle cells differentiated from the upper part of vibrissa hair follicles in culture medium that was non-supplemented (left panel); isoproterenol-supplemented (middle panel); and supplemented with the combination of isoproterenol+activin A+BMP4+bFGF (right panel). Cardiac muscle cells were observed after 14 days culture. Red = cTnT, Blue = DAPI. Bars = 100 µm.