Figure 2.

Flow cytometry to determine cTnT expression in HAP stem cell-derived cardiac muscle cells. (A) Flow cytometry analysis of cTnT-positive cardiac muscle cells that differentiated from the upper part of hair follicle cultured in medium that was non-supplemented at 8.78 ± 3.60% (left panel); in medium supplemented with isoproterenol alone at 19.71 ± 2.20% (middle panel); in medium supplemented with the combination isoproterenol, activin A, BMP4 and bFGF at 16.84 ± 1.67% (right panel). (B). The number of differentiated cTnT-positive cardiac muscle cells was significantly increased in the hair follicle cultured in medium supplemented with isoproterenol alone or the combination of isoproterenol, activin A, BMP4 and bFGF compared with unsupplemented medium.