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. 2016 Mar 30;15(5):654–666. doi: 10.1080/15384101.2016.1146839

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Figure 2. — In situ expression pattern of cdc-25.2 mRNA and partial rescue of the cdc-25.2 mutant phenotype by intestinal cdc-25.2 transgene expression. (A-F) In situ hybridization of cdc-25.2 during larval development with a cdc-25.2 sense probe (A, C and E) and a cdc-25.2 antisense probe (B, D and F): (A, B) L1 stage; broken lines in (B) indicate the region of cdc-25.2 mRNA expression, (C, D) L3 stage, (E, F) adult stage; cdc-25.2 mRNA expression was observed in the gonads. Scale bars, 25 μm in (B), 50 μm in (D), and 100 μm in (F). (G) GFP-marked intestinal nuclei in a Pelt-2::cdc-25.2 transgenic adult worm, in which a cdc-25.2 transgene under the control of the intestine-specific elt-2 promoter was expressed in a wild-type background. (H) GFP-marked intestinal nuclei in a cdc-25.2(ok597); Pelt-2::cdc-25.2 transgenic adult worm, in which the Pelt-2::cdc-25.2 transgene was expressed in the cdc-25.2 mutant background. Left, the anterior side. Scale bars, 50 μm. (I) Average numbers of intestinal nuclei in wild-type adults (n = 22), Pelt-2::cdc-25.2 adults (n = 19), cdc-25.2(ok597) adults (n = 23), and cdc-25.2(ok597); Pelt-2::cdc-25.2 adults (n = 30). ** p < 0.001.