Table 2.

Relative DNA content of int2 nuclei in respective genotypes.

Genotype	Relative DNA content of int2 nuclei	p value
wild type	1.0 ± 0.1 (n = 14)
cdc-25.2(ok597)	1.0 ± 0.1 (n = 14)	= 0.5
mock RNAi	1.0 ± 0.3 (n = 21)
cdk-1 RNAi	1.1 ± 0.3 (n = 19)	= 0.23
cdk-2 RNAi	0.6 ± 0.3 (n = 19)	< 0.05
cdk-4 RNAi	0.7 ± 0.4 (n = 13)	< 0.05
cyb-1 RNAi	1.1 ± 0.3 (n = 22)	= 0.48
cyd-1 RNAi	1.0 ± 0.2 (n = 16)	= 0.85
cye-1 RNAi	0.4 ± 0.2 (n = 24)	< 0.05

Intestine-specific RNAi was performed using the OLB11 strain. The relative DNA content of int2 nuclei in each genotype was determined as the average DNA staining intensity of int2 nuclei in each genotype normalized with that of int2 nuclei in the control (wild type or mock RNAi) measured at the adult stage. n, number of examined nuclei. P values are calculated against the appropriate controls.