Table 4.

RNAi depletion of genes encoding cell-cycle regulators, cdks, and cyclins in N2 and OLB11.

	Phenotype
Gene	N2	OLB11	Number of intestinal nuclei in OLB11
mock	wild type	wild type	29.7 ± 1.2 (n = 20)
cdc-25.1	Emb	wild type	31.6 ± 1.7 (n = 28)
cdc-25.2	Emb	number reduced	19.2 ± 5.3 (n = 29)**
cdc-25.3	wild type	wild type	32.1 ± 1.5 (n = 31)
cdc-25.4	wild type	wild type	29.8 ± 0.9 (n = 21)
wee-1.3	P0 sterile	wild type	33.3 ± 1.9 (n = 28)
cdk-1	1-cell Emb	number reduced	19.5 ± 1.6 (n = 24)**
cdk-2	Ste	number/DNA reduced	27.9 ± 1.9 (n = 34)**
cdk-4	Sck/ Dpy/ Lvl/ Emb	number/DNA reduced	26.8 ± 3.3 (n = 29)**
cdk-5	wild type	wild type	31.2 ± 1.6 (n = 29)
cdk-7	Slo/ Ste/ Lvl/ Emb	wild type	31.5 ± 1.2 (n = 32)
cdk-8	Ste	wild type	31.8 ± 1.6 (n = 16)
cdk-9	Emb	wild type	30.5 ± 1.4 (n = 25)
cyb-1	Emb	number reduced	25.1 ± 1.7 (n = 25)**
cyb-2.1	wild type	wild type	31.5 ± 1.7 (n = 25)
cyb-2.2	Lvl/ Emb	wild type	31.3 ± 1.5 (n = 27)
cyb-3	Emb	Emb	n.d.
cyd-1	Lvl	number reduced	25.1 ± 2.3 (n = 18)**
cye-1	Emb	number/DNA reduced	27.7 ± 2.4 (n = 20)*
cyh-1	Ste/ Emb	wild type	31.6 ± 1.5 (n = 25)
cyl-1	Emb	L3 arrest	27.0 ± 3.8 (n = 22)*

The indicated dsRNA solutions were injected into N2 or OLB11: rde-1(ne219) V; dusIs[Pelt-2::rde-1 + rol-6(+)] worms, and the resulting phenotype and the number of intestinal nuclei in OLB11 were examined in their progeny. Only the intestine is susceptible to RNAi in OLB11. Buffer injection was used as the mock control. Abbreviations used are as follows: Sck, sick; Dpy, dumpy; Ste, sterile; Lvl, larval lethal; Slo, slow growth; Emb, embryonic lethal; number reduced, the number of intestinal nuclei reduced; DNA reduced, the DNA content of intestinal nuclei reduced; number/DNA reduced, both the number and the DNA content of intestinal nuclei reduced; n.d., not determined.

^*p < 0.05,

^**p < 0.001.