Figure 4.

Tenovin-1 potentiated metformin-induced impair of mitochondrial function in C4-2 but not in LNCaP cells. (A, C) OCR under single or both drug treatment was measurement by Seahorse XFe²⁴ analyzer. Cells were seeded in XFe²⁴ cell culture microplates, treated with metformin, Tenovin-1 or both for 24 hours, and subjected to the protocol for mitochondria tolerance test in which oligomycin, FCCP and Rotenone/antimycin A were added at the time points indicated. (B, D) Calculated basal respiratory rate, spare respiratory capacity, proton leak and ATP production.