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. 2016 Apr 26;11(4):e0154027. doi: 10.1371/journal.pone.0154027

Table 4. Targeting multiple sites in rice using CRISPR/Cas9 and the number of plants with mutations at single, double and triple target sites.

No. of plants harboring mutations at target sites
Transformant No. of tested plants ERF922-S1 ERF922-S2 ERF922-S3 All*
pC-ERF922S1S2 30 21(70.0%) 19(63.3%) 19(63.3%)
pC-ERF922S1S2S3 30 27(90.0%) 27(90.0%) 27(90.0%) 27(90.0%)

* Based on the number of plants with mutations for all combinations of target sites.