Table 4. Targeting multiple sites in rice using CRISPR/Cas9 and the number of plants with mutations at single, double and triple target sites.
No. of plants harboring mutations at target sites | |||||
---|---|---|---|---|---|
Transformant | No. of tested plants | ERF922-S1 | ERF922-S2 | ERF922-S3 | All* |
pC-ERF922S1S2 | 30 | 21(70.0%) | 19(63.3%) | 19(63.3%) | |
pC-ERF922S1S2S3 | 30 | 27(90.0%) | 27(90.0%) | 27(90.0%) | 27(90.0%) |
* Based on the number of plants with mutations for all combinations of target sites.