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. 2016 Apr 15;9:2237–2245. doi: 10.2147/OTT.S100952

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© 2016 Fu et al. This work is published and licensed by Dove Medical Press Limited

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PER1 mRNA and protein expression in SCC15 cells.

Notes: (A) PER1 mRNA expression levels were determined by quantitative real-time PCR. (B) Representative PER1 protein expression was determined by Western blot analysis, and (C) the band density values were calculated as a ratio to GAPDH. ^***P<0.0001 and ^*P<0.05 compared to control cells. The data are presented as the mean ± SD, and the results were analyzed by one-way ANOVA with pairwise comparisons, followed by the LSD-t.

Abbreviations: PCR, polymerase chain reaction; SD, standard deviation; ANOVA, analysis of variance; LSD-t, least significant difference test; shRNA, short hairpin RNA; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.