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. 2016 Apr 19;9:39–50. doi: 10.2147/JIR.S100940

Figure 3.

Figure 3

Ixekizumab neutralizes human IL-17A, human IL-17A/F heterodimer, and cynomolgus monkey IL-17A.

Notes: (A) HT-29 cells were treated with a dose range of human IL-17A or IL-17A/F for ∼48 hours. GROα was measured in the culture medium by ELISA. (B) HT-29 cells were treated with a constant amount of either human IL-17A (60 ng/mL =1,875 pM) or human IL-17A/F (1,000 ng/mL =32,573 pM), in the presence of either ixekizumab or control human IgG4 at the indicated concentrations. After ∼48 hours, GROα in the culture media was measured by ELISA. (C) HT-29 colon cancer cells were treated with a constant amount of cynomolgus monkey IL-17 (60 ng/mL), in the presence of either ixekizumab or control human IgG4 at the indicated concentrations for 48 hours, and GROα in the culture media measured by ELISA. Results in (A) and (B) are shown as the mean of triplicate treatments ± SD and are representative of seven independent experiments. Results in (C) are shown as the mean of triplicate treatments ± SD and are representative of two independent experiments. Human and cynomolgus monkey IL-17A were tested for neutralization in separate experiments.

Abbreviations: cyno, cynomolgus; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; SD, standard deviation; GRO, human growth-regulated oncogene.