Figure 1.

Carvacrol suppresses proliferation in human tongue cancer cells.

Notes: (A) Chemical structure of carvacrol (5-isopropyl-2-methylphenol). Tca-8113 cells (B) and SCC-25 cells (C) were cultured with the indicated concentrations of carvacrol in 96-well plates. After 24 hours (h), the cells were treated with MTT for 4 hours, and absorbance was measured. Results represent the mean ± standard deviation of three experiments done in triplicate. *P<0.05, **P<0.01 vs the Tca-8113 control group. (D) Carvacrol reduces s-phase of Tca-8113 cells compared to control cells. (E, F) Tca-8113 cells were treated with various concentrations of carvacrol (0~80 µM) for 24 hours and then examined for expression of P21, CCND1, CDK4, and the unchanged expression of CDK2 and CDK6 by Western blot analysis. β-actin was used as an internal control for equal amount of total proteins. (G) Colony formation assay of Tca-8113 cells.

Abbreviation: DMSO, dimethyl sulfoxide.