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. 2016 Apr 20;9:2393–2402. doi: 10.2147/OTT.S91097

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© 2016 Liu et al. This work is published and licensed by Dove Medical Press Limited

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The silencing efficiency of SPOP in A498 and ACHN cells.

Notes: (A and C) The mRNA level of SPOP after transfection with siRNA-SPOP was detected by qPCR in the A498 and ACHN RCC cells, respectively. (B and D) The protein level of SPOP after the transfection with siRNA-SPOP was detected by Western blot in the A498 and ACHN cells. *P<0.05 vs control and ^#P<0.05 vs NC. Control group: nontreatment group; NC group: transfection with nonspecific siRNA-SPOP.

Abbreviations: SPOP, speckle-type POZ protein; siRNA, small interference RNA; qPCR, quantitative real-time polymerase chain reaction; RCC, renal cell cancer; NC, negative control; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.