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. 2016 Apr 26;11(4):e0154285. doi: 10.1371/journal.pone.0154285

Fig 1. Purity and antigenicity of recombinant GST-MDA5 protein.

Fig 1

A. Purified recombinant GST-MDA5 fusion protein (2 μg) was subjected to sodium dodecyl sulfate-12.5% polyacrylamide gel electrophoresis, followed by 0.05% Coomassie blue staining. The GST-MDA5 fusion protein was detected as a single band at ~166 kDa (arrow). B. Immunoblot analysis of the recombinant fusion protein was performed by probing with anti-MDA5 antibody-positive DM sera (lanes 1–3), anti-MDA5 antibody-negative DM sera (lanes 4–6), an anti-GST polyclonal antibody (lane 7), and an anti-MDA5 monoclonal antibody (lane 8).