Fig 1.
a) Expression of VirB9-1 protein. L: ladder marker; P: uninduced total E. coli cell lysate; T: induced total E. coli cell lysate; S: supernatant of E. coli cell lysate after centrifugation at 27,000 g, 4°C for 5 minutes. 6 μL of the ladder was loaded, while the other samples were all 8 μL. GST-tagged VirB9-1 protein is approximately 56 kDa as labelled. b) Expression of VirB9-2 protein. Top: uninduced sample; Bottom: induced sample. L: ladder marker; S: soluble fraction; P: pellet fraction; 0-S: 0 h supernatant; 0-P 0 h pellet; 4-S: 4 h supernatant; 4-P: 4 h pellet. VirB9-2 protein is approximately 44 kDa as labelled.
