Table 1. M. extorquens strains, plasmids and primers used in this study.

Strains	Genotype or relevant features	Source or reference
Wide Type	Rifamycin-resistant strain	[2]
NG107	pCM80	[26]
NG115	pCM80 (PmaxF::ecm)	[26]
JY213	pCM80 (PmaxF::phaA)	this work
JY214	pCM80 (PmaxF::mcmA)	this work
JY215	pCM80 (PmaxF::mcmB)	this work
Plasmids	Description
pCM80	M. extorquens expression vector, mxaF promoter; TcR	[31]
pCM80-ecm	pCM80 containing ecm gene from M. extorquens	[26]
pCM80-phaA	pCM80 containing phaA gene from M. extorquens	this work
pCM80-mcmA	pCM80 containing mcmA gene from M. extorquens	this work
pCM80-mcmB	pCM80 containing mcmB gene from M. extorquens	this work
Primers	Sequence (5’–3’)
phaA_pCM80_fw	ctcgagAAGCTT ATGGCAGCCAGTGAAGATATCGTC	this work
phaA_pCM80_rev	ctcgagGGATCC TCAGACCCGCTCGACACACATG	this work
mcmA_pCM80_fw	ctcgagAAGCTT ATGAGCTCCCGCATCCCCGATTTC	this work
mcmA_pCM80_rev	ctcgagGGATCC CTACTCGGCCGCCTGCCGTTC	this work
mcmB_pCM80_fw	ctcgagAAGCTT ATGGAAGATCGTCCGCTCGCCGA	this work
mcmB_pCM80_rev	ctcgagGGATCC GCGGCCTTGAGTGTTAGTCAGCC	this work