Figure 1.

Loss of CD151 or CD151-integrin interaction reduces the tightness of cell-cell junctions. (A-E) Electron micrographs of cell-cell junctions within monolayers of A431 parental cells (WT), CD151sh3 cells, CD151-VR cells, CD151-Palm cells, or CD151 WTRX cells. The rectangular area in each micrograph was zoomed and rotated to vertical to the right of each image. (F) The percentage of each junction filled with cross interacting membrane and filopodia was calculated as described in Experimental Procedures. Bars show mean with SEM for 13–17 fields per cell type. The sh3 and VR cell junctions were significantly less densely packed than the WT cell junctions, ***P ≤ 0.0006 (ANOVA with Dunnett's post-test). Bar = 1 µm.