Figure 4.
CDK10/CycM maintains RhoA stability through PKN2 phosphorylation (A-D) Western blot analysis (A, C) and quantification using imageJ (B, D) of endogenous RhoA protein levels in serum-starved hTERT-RPE1 cells: (A, B) without (empty vector) or with ectopic expressed of Myc-PKN2WT or Myc-PKN2TATA; or (C, D) without (siCTL) or with CDK10/CycM or PKN2 silencing (as described in the prior figure legends). RhoA protein levels were normalized on GAPDH protein levels. (E) Quantification of RhoA RNA levels in different conditions described above, using quantitative RT-PCR. RhoA RNA levels were normalized on GAPDH RNA levels. n.s. = not significant.