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. 2016 Mar 30;15(5):678–688. doi: 10.1080/15384101.2016.1147632

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Figure 5. — CDK10/CycM represses ciliogenesis through the modulation of RhoA signaling (A) Immunofluorescent visualization of Actin (Rhodamin-Phalloidin staining, shown in red), DNA (DAPI staining, shown in blue) and pMLC2 (shown in green) in serum-starved hTERT-RPE1 cells without (siCTL) or with CDK10/CycM silencing. The arrowhead indicates a cell with diffuse pMLC2 staining and reduced stress fibers. The arrow indicates a cell lacking pMLC2 staining and stress fibers. Scale bar, 25 μm. (B) Western blot analysis of exogenously-expressed Flag-RhoA protein levels in serum-starved hTERT-RPE1 cells, without (siCTL) or with CDK10/CycM silencing. (C) Percent ciliated cells (quantified as described in Fig. 1 legend) in the absence and presence of Flag-RhoA expression and CDK10/CycM silencing. n.s. = not significant.